Background
Organisms, when being invaded by foreign invaders, produce an antimicrobial agents to battle the attackers. The goal of this project was to isolate the antimicrobial agents in the plants that we tested on. Once the samples are collected, samples can be tested for their ability to kill different microbes. After, scientists must prove that they won’t cause negative effects in humans.
Purpose
What plant materials, found locally, contain active ingredients that will inhibit the growth of bacteria?
Materials
Procedure
Results
Table in document below.
Analysis/Conclusions
When examining our disks, we realized that we had a case of bacterial contamination. In our 48 hour H20 disk, there is there were different shapes and colors that represented different kinds of bacteria. As for our methanol disk, the bacterial lawn did not grow properly and if there was a clearance, it was not possible to see it. The results of our project are inconclusive and if rosemary plant can be used as a medicine, we did not find it here.
Organisms, when being invaded by foreign invaders, produce an antimicrobial agents to battle the attackers. The goal of this project was to isolate the antimicrobial agents in the plants that we tested on. Once the samples are collected, samples can be tested for their ability to kill different microbes. After, scientists must prove that they won’t cause negative effects in humans.
Purpose
What plant materials, found locally, contain active ingredients that will inhibit the growth of bacteria?
Materials
- Balance
- Weigh Boat
- Lab Scoops
- LB Broth Base
- Media Bottles
- 250 mL Sterilizer/Autoclave
- Water Bath (37 Degrees)
- Sterile LB Agar
- Rosemary Plant
- Funnel
- Coffee Filters
- Test Tube Holder
- Alcohol
- Petri Dishes
- Mortar & Pestel
- 10 mL Water
- 10 mL Methanol
- Rack
Procedure
- Sterile disks were added to the microfuge tubes containing 1 mL of sterile water and 1 mL of ampicillin.
- 10-20 mL of warmed nutrient agar was poured into 2 petri dishes using sterile techniques.
- After allowing agar to solidify, plates were turned upside down and stored at 4 degrees Celsius overnight.
- 1 mL of E-Coli colony was added to each plate. A flame-sterilized spreading loop was used to spread the bacteria throughout the surface of the agar.
- Using flame-sterilized forceps, filter disks were placed in separate quadrants onto the plate in the following sequence: 1. Water, 2. Plant Extracts, 3. Ampicillin. The plates were left on lab bench for 20 minutes to allow both bacteria and filter disks to adhere to the agar.
- Plates were incubated upside down, overnight at 37 degrees Celsius. Plates were photographed and observed for clearance around the filter dishes after 24 hours, 48 hours, and 72 hours.
Results
Table in document below.
Analysis/Conclusions
When examining our disks, we realized that we had a case of bacterial contamination. In our 48 hour H20 disk, there is there were different shapes and colors that represented different kinds of bacteria. As for our methanol disk, the bacterial lawn did not grow properly and if there was a clearance, it was not possible to see it. The results of our project are inconclusive and if rosemary plant can be used as a medicine, we did not find it here.